Advanced Instrumentation Techniques | Best B pharmacy Semester 8 free notes | Pharmacy notes pdf semester wise

Semester 8

BP 811 ET. ADVANCED INSTRUMENTATION TECHNIQUES

Scope: This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart advanced knowledge on the principles and instrumentation of spectroscopic and chromatographic hyphenated techniques. This also emphasizes theoretical and practical knowledge of modern analytical instruments that are used for drug testing. 

Objectives: Upon completion of the course the student shall be able to

•  understand the advanced instruments used and its applications in drug analysis 
• understand the chromatographic separation and analysis of drugs. 
• understand the calibration of various analytical instruments 
•  know analysis of drugs using various analytical instruments.

Course Content:

UNIT-I

Nuclear Magnetic Resonance spectroscopy

Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications

Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

UNIT-II

Thermal Methods of Analysis: Principles, instrumentation and applications of thermogravimetric analysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)

X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X-ray Crystallography, rotating crystal technique, single-crystal diffraction, powder diffraction, structural elucidation and applications. 

UNIT-III

Calibration and validation-as per ICH and USFDA guidelines 

Calibration of following Instruments

Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer,

Fluorimeter, Flame Photometer, HPLC and GC

UNIT-IV

Radio immune assay: Importance, various components, Principle, different methods, Limitation, and Applications of Radio immunoassay

Extraction techniques: General principle and procedure involved in the solid-phase extraction and liquid-liquid extraction

UNIT-V

Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. 

Recommended Books (Latest Editions)

1. Instrumental Methods of Chemical Analysis by B.K Sharma

2. Organic spectroscopy by Y.R Sharma

3. Textbook of Pharmaceutical Analysis by Kenneth A. Connors

4. Vogel’s Textbook of Quantitative Chemical Analysis by A.I. Vogel

5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake

6. Organic Chemistry by I. L. Finar

7. Organic spectroscopy by William Kemp

8. Quantitative Analysis of Drugs by D. C. Garrett

9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi

10. Spectrophotometric Identification of Organic Compounds by Silverstein